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. 2017 Oct 26;7(1):1386536. doi: 10.1080/20008686.2017.1386536

Table 2.

The outcome of the different DNA extraction kits and their alterations tested in the current study.

ID Kit name NanoDrop ng/μL A260/A280 A260/A230 QC gel electrophoresis 16S PCR gel
E1 PowerSoil DNA Isolation Kit 5.70 2.08 0.685 Empty N/A
E2a Maxwell 16 Tissue DNA Purification Kit 163a 0.695 0.200 Faint smear high-weight fragments
E3 6.30 3.31 0.990 Faint smear middle-weight fragments
E4 DNeasy Blood & Tissue Kit 4.75 0.625 0.840 Empty
E5 16.0 1.51 0.175 Faint smear middle-weight fragments
E6 QIAamp Fast DNA Stool Mini Kit 0 N/A N/A Empty Empty
E7 1
E8 QIAamp DNA Stool Mini Kit 1
E9 9.90 0.475 0.4825 N/A
E10 4.35 2.12 0.940
E11 QIAamp cador Pathogen Kit 10.3 4.82 0.460 Faint smear high-weight fragments 30 cycles faint bands 100–(−2) dilution
E12 75.3 1.20 0.815 Strong smear high-weight fragments 30 cycles strong bands 10(−1)–(−2) dilution
E13 17.4 1.58 0.385 N/A 35 cycles 10(−2) dilution weak bands, 10(−3) dilution strong bands
E14 77.1 0.795 0.350 35 cycles 10(−2) dilution strong bands, 10(−3) dilution weak bands
E15 44.1; 8.40 1.63; 1.67 0.810; 1.10

aVery heavy bead carryover interfering with eluate quantification.

The sign " denotes that the value/setting was identical to the one given directly above.