Table 2.
ID | Kit name | NanoDrop ng/μL | A260/A280 | A260/A230 | QC gel electrophoresis | 16S PCR gel |
---|---|---|---|---|---|---|
E1 | PowerSoil DNA Isolation Kit | 5.70 | 2.08 | 0.685 | Empty | N/A |
E2a | Maxwell 16 Tissue DNA Purification Kit | 163a | 0.695 | 0.200 | Faint smear high-weight fragments | “ |
E3 | “ | 6.30 | 3.31 | 0.990 | Faint smear middle-weight fragments | “ |
E4 | DNeasy Blood & Tissue Kit | 4.75 | 0.625 | 0.840 | Empty | “ |
E5 | “ | 16.0 | 1.51 | 0.175 | Faint smear middle-weight fragments | “ |
E6 | QIAamp Fast DNA Stool Mini Kit | 0 | N/A | N/A | Empty | Empty |
E7 | “ | 1 | “ | “ | “ | “ |
E8 | QIAamp DNA Stool Mini Kit | 1 | “ | “ | “ | “ |
E9 | “ | 9.90 | 0.475 | 0.4825 | “ | N/A |
E10 | “ | 4.35 | 2.12 | 0.940 | “ | “ |
E11 | QIAamp cador Pathogen Kit | 10.3 | 4.82 | 0.460 | Faint smear high-weight fragments | 30 cycles faint bands 100–(−2) dilution |
E12 | “ | 75.3 | 1.20 | 0.815 | Strong smear high-weight fragments | 30 cycles strong bands 10(−1)–(−2) dilution |
E13 | “ | 17.4 | 1.58 | 0.385 | N/A | 35 cycles 10(−2) dilution weak bands, 10(−3) dilution strong bands |
E14 | “ | 77.1 | 0.795 | 0.350 | “ | 35 cycles 10(−2) dilution strong bands, 10(−3) dilution weak bands |
E15 | “ | 44.1; 8.40 | 1.63; 1.67 | 0.810; 1.10 | “ | “ |
aVery heavy bead carryover interfering with eluate quantification.
The sign " denotes that the value/setting was identical to the one given directly above.