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. 2017 Nov;58(13):5654–5665. doi: 10.1167/iovs.17-22071

Figure 3.

Figure 3

Expression of Panx1, Panx2, and proinflammatory and differentiation factor mRNAs after LG injury and during LG regeneration. Graphic representation of the Table. LGs were injected with IL-1 or saline (vehicle control) and collected at days 1, 2, 3, 7, and 21 after injection for RNA extraction. Levels of mRNAs were determined by qRT-PCR. (A) Expressions of Panx1 and purinergic receptors P2RX7 and P2RY2 were upregulated during first 1 to 3 days after the LG injury (inflammation stage), whereas expressions of Panx2 and collagens I and XVI (A: Col16a1 and Col1a1) were increased during the regeneration phase, on days 5 to 7 after IL1β injection. Expressions of several inflammasome related factors (B, C: NLRP3, Casp1, Casp4 [11] and Casp8); proinflammatory cytokines (C: IL1b, IL6, IL18), and IL1 family member 9 (C: Il1f9, proinflammatory stimulator of chemokine); and specific proinflammatory chemokines (D: Ccl2, Ccl6, Ccl7, and Ccl12) were significantly increased at days 1 to 3 after LG injury.