Figure 4.

Pharmacologic blockade of Panx1 increases engraftment of transplanted EPCPs into the injured host LG. (A) Experimental paradigm. Recipient LGs were injected with IL1α alone or combined with a mimetic peptide inhibitor of Panx1 ¹⁰panx (100 mM) or Panx1 sdRNAi and donor cells, sorted from R26-LacZ mouse, and were transplanted 1 day after injury and analyzed 40 days after transplantation. (B) Blocking Panx1 channels by ¹⁰panx or Panx1 sdRNAi increased the engraftment of EPCP cells relative to control LGs. Engraftment of transplanted EPCPs into control (C) or sdRNAi-treated LGs (D) (black arrows, LacZ⁺ engrafted cells. In (B) *P < 0.01.