Fig. 3.

p38 MAPK pathway affected hepatocytes differentiation associating with downregulation of Cx43 and upregulation of Cx32. a Western blotting of phospho-p38 (P-p38) and total p38 in WB cells and WB-Hep. b qRT-PCR analysis of WB-Hep induced with or without SB203580 treatment. c Western blotting of WB-Hep induced with or without SB203580 treatment. d qRT-PCR analysis of WB-Hep induced with SB203580 from WB cells and Cx43-overexpressed WB cells. e Western blotting of WB-Hep induced with SB203580 from WB cells and Cx43-overexpressed WB cells. f Cell viability test by CCK8. g Flow cytometry analysis of apoptosis. h ICG uptake. i Induction of CYP3A4 activity in response to 72 hours of stimulation with PXR agonist rifampicin. Data represented as mean ± SEM. ^* p < 0.05, ^** p < 0.01