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. 2017 Nov 7;17:719. doi: 10.1186/s12885-017-3701-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The cellular morphology was changed when E2F1 was depleted in SCLC cells. a E2F1 protein was examined in 8 clones where E2F1 was knocked down by shRNA in H446 cells (H446-E2F1sh) and transiently silenced by E2F1 specific siRNA in H1688 cells (H1688-E2F1si). b Cellular morphology was changed from grain-shaped to slender and fibrous in clone-4 of H446-E2F1sh and H1688-E2F1si cells. Because shRNA vector contained eGFP, H446-E2F1sh cells were green. The magnification of H446 (panel b) was 400 X, and the magnification of H1688 (panel c) was 200 X