Fig. 7.

E2F1 could regulate ZEB2 expression in SCLC cell lines. a The expression levels of ZEB2, SNAI1 and SNAI2 mRNA were detected by real-time PCR in H446 and H1688 cells. ZEB2 protein was detected in H446-E2F1sh and H1688-E2F1si cells. mRNA levels were calculated in 3 independent experiments. * represents p < 0.05. b ZEB2 was highly expressed in tissue samples in which E2F1 was also high. Representative image was presented, and the magnification is 200 X. c Dual luciferase activity analysis from 3 independent experiments showed that E2F1 could directly regulate ZEB2 expression in H446 and H1688 cells, * represents p < 0.05, ** represents p < 0.001. RLA: Relative luciferase activity