Figure 4.

Semiquantification of immunogold-labeled albumin-like protein in proximal tubules of zebrafish. (A) Bar graph illustrates semiquantitative data of protein uptake in proximal tubules. Statistically, (b) denotes results significantly different from controls (P < 0.01). (B) Immunoelectron microscopy images showing gold-labeled albumin-like protein (arrowheads) in controls (a, b) and in nphs2 morphants injected with 100 μM of MO (c, d) in the brush border (left panel) and cytoplasmic vesicles (right panel), respectively (×60,000). Bar = 200 nm. MO, morpholino antisense oligo.