Figure 5.

MALAT1 promotes proliferation, invasion and EMT of NPC cells by sponging miR-124. 5–8F and HONE-1 cells were introduced with miR-124 alone, or in combined with pcDNA-MALAT1. (A and B) MTT assay was performed to detect cell viability at 24, 48 and 72 h after transfection. (C and D) Transwell chamber assay was conducted to determine cell invasion at 48 h after transfection. (E and F) Expression of E-cadherin, N-cadherin and vimentin was measured by western blot analysis in 5–8F and HONE-1 cells 48 h post-transfection. **P < 0.01, ***P < 0.001 vs. controls.