Figure 6.
MALAT1 promotes Capn4 expression by sponging miR-124 in 5–8F and HONE-1 cells. (A) 3′ UTR binding sites of Capn4 within miR-124 are shown. (B and C) The relative luciferase activity was measured in 5–8F and HONE-1 cells co-transfected with Capn4-WT or Capn4-Mut reporter and miR-124 mimic or miR-124 mimic + pcDNA-MALAT1. (D and E) Western blot was performed to detect the Capn4 expression in 5–8F and HONE-1 cells transfected with si-MALAT1, pcDNA-MALAT1, miR-124 or co-transfected with miR-124 and pcDNA-MALAT1. **P < 0.01, ***P < 0.001 vs. controls.