Capn4 overexpression abrogates the inhibitory effect of MALAT1 knockdown on proliferation, invasion and EMT of NPC cells. 5–8F and HONE-1 cells were introduced with si-MALAT1 or co-transfected with si-MALAT1 and pcDNA-Capn4. (A and B) MTT assay of viability in 5–8F and HONE-1 cells at 24, 48 and 72 h post-transfection. (C and D) Transwell chamber assay of invasive ability in 5–8F and HONE-1 cells 48 h after transfection. (E and F) Western blot analysis of E-cadherin, N-cadherin and vimentin in 5–8F and HONE-1 cells 48 h after transfection. *P < 0.05, **P < 0.01, ***P < 0.001 vs. controls.