Figure 6.

AHCC upregulates miR-335 expression in MDA-MB-231 mammospheres. Results are a combination of means ± SEM from 3 separate experiments. MDA-MB-231 cells were plated in 6-well ultra-low attachment plates in DMEM-F12 and spheroid medium and incubated at 5% CO₂ and 37°C. Cells were exposed to varying concentrations of AHCC for 5 hours. MiR-335 expression levels were measured by RT-qPCR in comparison to reference snRNA U6. Significance is represented by * for p < 0.05 by Tukey's post-hoc test.