Figure 5.

The efficacy of Shh/RhoA pathway on tube formation of HUVECs in vitro. A, Phase-contrast micrographs showed that MVs harboring Shh enhanced network formation of HUVECs on Matrigel. The efficacy of MVs-enhanced network formation was inhibited by C3 transferase (5 ug/ml). The Shh (5 ug/ml) was used as the positive control. The treatment condition and the actual number of branch points ± SEM were underneath the image. Branch points were used to quantify angiogenesis; B, Shh associated tube formation was related with RhoA activation; C, the expression of Gli1 in HUVECs after rh-shh (5 ug/ml) treatment. A difference of P < 0.05 was considered significant.