Fig. 5.

MiR-214-3p down-regulates MELK expression. a WT and MUT of putative miR-214-3p target sequences of MELK 3′-UTR; b WT and MUT miR-214-3p target sequences were cotransfected with miR-214-3p precursor into HEK293T cells.; c Effects of miR-214-3p overexpression on the expression level of MELK in HepG2 and HUH-7 cells transfected with miR-214-3p by Western blot; d Expression of MELK and miR-214-3p in HCC tissues detected. Protein expression of MELK in HCC samples 1 and 2 was detected by IHC (× 200) while expression of miR-214-3p was determined using real-time PCR. 1, 2 refer to HCC sample 1 and 2. (*P < 0.05; Student’s t-test)