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. 2017 Sep 6;18(10):801–805. doi: 10.1080/15384047.2017.1373216

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© 2017 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 2. — Validation of miR-129-2-based regulation of PR. (a) pCDNA3.1-miR-129-2 and pGL3- PR 3′UTR in different combinations with anti-miR-129-2 were co-transfected in 293FT cells and luciferase signal in each condition was measured, as shown in the figure. (b) Quantified luminescence units normalized to renilla expression was plotted for each of the sets mentioned above. Analysis is representative of three independent experiments and the P-value was calculated using student's t-test. ** indicates P-value <0.001; *** indicates P-value <0.0001.