Figure 4.

KLF5-downregulating CAFs inhibit tumor cell migration and invasion by reducing CCL5 secretion (A) Representative images of cytokine expression difference between CAFs-CM and CAFs-KLF5-si-CM in human cytokine antibody array. (B) Cytokine screening of conditioned medium collected from CAFs and CAFs-KLF5-si. (C) qRT-PCR detection of CCL5 mRNA levels in four pairs of CAFs and NFs. (D) ELISA detection of CCL5 protein concentration in the medium conditioned by these cells. (E, F) Transwell invasion and wound-healing assay showed the effect of different treated CAFs on tumor cell invasion in the presence of CCL5 neutralizing antibody or exogenous CCL5. Data are representative of three independent experiments. *P < 0.05 by t test, **P < 0.01 by t test.