Fig. 5.

p53 regulated GSTP1 expression and chemoresistance in MCF-7 cells. a p53 expression when CLDN6 overexpression in MCF-7 cells was measured by western blot. b Three different TP53 specific shRNA constructs were utilized to screen the optimal sequence in MCF-7/CLDN6 cells by using western blot. c qPCR was applied the mRNA expression of GSTP1 when silenced TP53 gene in MCF-7/CLDN6 cells. d GSTP1 expression was observed when silenced TP53 gene by using western blot in MCF-7/CLDN6 cells. e GST activity was measured when inhibited TP53 gene expression. f IC₅₀ of ADM and 5-FU when inhibited TP53 in MCF-7/CLDN6 cells. g Western blot analysis p53 expression in MCF-7/MDR cells when silenced CLDN6 gene. *, P < 0.05; **, P < 0.01