Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 7;8:256. doi: 10.1186/s13287-017-0707-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 3 — Colony forming efficiency (CFE) and corneal epithelial cell marker expression from the three cultures. a, b Crystal violet staining of LESC colonies from the three cultures. Red arrows point to holoclones. Green arrows point to meroclones. Black arrows point to paraclones. CFE of holoclones from the three cultures were quantified. c, d Corneal epithelial differentiation marker CK12 staining of the three cultures. Percentage of CK12⁺ cells were quantified. e, f Corneal epithelial tight junction protein Claudin-1 staining of the three cultures. Percentage of Claudin-1⁺ cells were quantified. Data were shown as mean ± SD from three independent experiments. One-way ANOVA analysis: *P < 0.05; ***P < 0.001. Scale bar, 50 μm. CK12 cytokeratin12, DAPI 4′,6-diamidino-2-phenylindole