Table 2.
Validation of microarray results in tumour samples
| microRNA | microarray p-value | RT-qPCR p-value |
Average RT-qPCR repeats, normalized | |||
|---|---|---|---|---|---|---|
| Control | Benign | Malignant non-metastatic |
Malignant metastatic | |||
| let-7c | < 1e-07 | 0.02892256 | 2.066592593 | 1.551592593 | 0.885092593 | 0 |
| miR-26b | < 1e-07 | 0.00001507 | 1.56060648 | 0.04060648 | −0.57989352 | −1.02131944 |
| miR-26a | < 1e-07 | 0.00003571 | 1.11365741 | 0.50621296 | −0.58834259 | −1.03152778 |
| miR-30a | 3.00E-07 | 0.00367713 | −0.09936889 | 0.71563112 | 0.08413112 | −0.70039334 |
| miR-148a | 3.00E-07 | 0.04282469 | 0.32479167 | 0.55945833 | −0.50670833 | −0.37754167 |
| miR-29c | 5.00E-07 | 0.00019157 | 0.79212037 | 0.30412037 | −0.25387963 | −0.84236111 |
| miR-30c | 2.70E-06 | 0.00133947 | 0.2615463 | 0.59621296 | 0.0315463 | −0.88930556 |
| miR-299 | 5.40E-06 | 0.04632324 | 1.899074074 | 0.425074074 | 2.399074074 | 0 |
| miR-10b | 6.00E-06 | 0.01635353 | 0.9961408 | 0.07058524 | −0.46735921 | −0.59936683 |
| miR-30b | 1.74E-05 | 0.00557426 | 0.61328913 | 0.43528913 | −0.40171087 | −0.6468674 |
one-way ANOVA p-values for microarray results and RT-qPCR results; RT-qPCRs performed for 10 microRNAs on 47 samples (5 controls, 5 benign tumours, 10 malignant non-metastatic tumours and 27 malignant metastatic tumours)