Table 3.
Validation of selected targets for microRNAs deregulated in metastatic canine mammary cancer
| Gene | Mean difference | Lower | Upper | p-value |
|---|---|---|---|---|
| CDC6 | 1.662166667 | 0.296219571 | 3.028113763 | 0.014739925 |
| CCNE1 | 1.805333334 | 0.698148282 | 2.912518385 | 0.00111525 |
| MYBL2 | 1.469333333 | 0.095498643 | 2.843168024 | 0.034267 |
| PDCD10 | 0.315 | −1.466393723 | 2.096393723 | 0.899858502 |
| ERBB2IP | 1.863333333 | 0.181873946 | 3.544792721 | 0.027609459 |
| SON | 1.691333333 | 0.149694553 | 3.232972114 | 0.029382504 |
| STK4 | 2.356333333 | 0.708656745 | 4.004009921 | 0.004014445 |
| CDC27 | 2.316333334 | 0.684213855 | 3.948452812 | 0.004297527 |
| PRC1 | 3.368333333 | 1.644772232 | 5.091894434 | 0.000132898 |
| CDC37 | 3.250833333 | 1.273918023 | 5.227748644 | 0.001019454 |
| TTK | 2.8475 | 0.856445769 | 4.838554231 | 0.004013127 |
| SKIL | 2.291666667 | 0.327926258 | 4.255407076 | 0.019726893 |
| BUB3 | 0.976666667 | −0.467839044 | 2.421172377 | 0.232448632 |
| SPIN1 | 1.248333333 | −0.448209573 | 2.944876239 | 0.180817071 |
The analysis performed on ten malignant non-metastatic tumours and ten malignant metastatic tumours. Statistical analysis was made on Ct values normalized with a housekeeping gene; one-way ANOVA followed by Tukey’s HSD post hoc tests