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. 2016 Mar 14;3:64–67. doi: 10.1016/j.meteno.2016.03.001

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3. — Improved induction of hCLCA2 after Na Butyrate treatment. A) Immunoblot of flag-tagged hCLCA2 (H2-flag) Tripz-transduced HMLE. X, nonspecific band serves as loading control. B) Immunofluorescence of doxy-induced cells treated with 1 mM Na Butyrate and probed with anti-Flag and Alexa568-labeled secondary antibody. C) Immunoblot of hCLCA2-flag-Tripz transduced MDA-MB-231 cells. D) Enhancement of transcriptional induction in cells from C. Cells were treated with 1 µg/ml doxycycline. Induced mRNA was measured by RT-qPCR and normalized to beta actin mRNA. In A and C, protein was immunoprecipitated with anti-Flag antibody M2 before electrophoresis.