Table 6.

Metabolite production from glucose bio-conversion in micro-aerobic cultures of IMZ500 (Δpdc1,5,6 MTH1ΔT p426GPD) and IME307 (Δpdc1,5,6 Δilv2 MTH1ΔT^coilvBCDN^cokdcA). Cells were first grown in SMG medium in aerobic, pH-controlled bioreactors, then washed with water and resuspended to a final cell density of ~12 g/L in SMG medium supplemented with Tween-80 (420 mg/L) and ergosterol (10 mg/L) with the initial pH set to 6.0. Cells were then incubated micro-aerobically at 30 °C and metabolite concentrations were measured during linear glucose consumption. Data are presented as average and mean deviation of duplicate experiments. *Sum total of extracellular diacetyl and acetolactate, BD: Below detection limit of analytical methods, NA: Not applicable, ND: Not determined, DHIV: 2,3-dihydroxy-isovalerate, KIV: α-keto-isovalerate.

	IMZ500		IME307
	Rate	Yield	Rate	Yield
	(µmol/g biomass/h)	(mol/mol glucose)	(µmol/g biomass/h)	(mol/mol glucose)
Glucose	−155.74±11.00	NA	−25.95±4.57	NA
Pyruvate	116.66±0.94	0.770±0.101	1.08±0.28	0.041±0.013
Ethanol	BD	BD	3.37±0.75	0.131±0.039
Glycerol	123.42±25.36	0.76±0.00	11.73±3.41	0.51±0.00
Acetoin	BD	BD	BD	BD
2,3-butanediol	BD	BD	13.66±1.70	0.649±0.067
DHIV	BD	BD	1.71±0.30	0.070±0.000
KIV	BD	BD	BD	ND
Isobutyrate	BD	BD	1.40±0.12	0.065±0.005
Isobutanol	BD	BD	0.41±0.09	0.018±0.003