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. 2017 Nov 8;12(11):e0187194. doi: 10.1371/journal.pone.0187194

Fig 1. Increase mRNA levels of human gal-7 in cancer cells following stimulation with human recombinant galectin-7 (rhGal-7).

Fig 1

(A) Levels of transcripts were measured by RT-PCR 16 h after addition of rhGal-7. (B) Kinetic analysis of lgals7 mRNA expression induced by rhGal-7 (5μM) in OVCAR and A2780 cells. (C) mRNA level of lgals7 in MDA-MB-231 cells after treatment with rhGal-7 (5μM). (D) Quantitative analyses of lgals7 mRNA levels in MDA-MB-231 as measured by imaging densitometry. (* p ≤ 0.05). (E) Luciferase activity measured in protein extracts collected from MDA-MB-231 cells transfected with a luciferase reporter vector containing p200-gal7 promoter following treatment with rhGal-7 (** p ≤ 0.005). (F) lgals7 expression induced by rhGal-7 at different concentrations. Data are representative of at least three independent experiments.