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. 2017 Nov 8;12(11):e0187732. doi: 10.1371/journal.pone.0187732

Fig 2. Wavelength-selective photostimulation of AMPA and GABA-A receptors in hippocampal CA1 neurons.

Fig 2

(a) Currents induced by near-UV and blue light in a recirculating solution of dcPNPP-Glu. Left, example traces of currents induced by near-UV (top) and blue (bottom) light. Right, increasing the duration of near-UV light flashes increased the amplitude of the inward current that was power dependent (n = 3 cells). Blue light was ineffective at all flash durations. (b) Currents induced by blue and near-UV light in a recirculating solution of DEAC454-GABA. Left, example traces of currents induced by blue (top) and near-UV (bottom) light. Right, Summary of results using different duration flashes of blue and near-UV light at two different powers (n = 3 cells). (c) Plots illustrating the power window of dcPNPP-Glu and DEAC454-GABA of data shown in (a/b). Left, there is no functional cross-talk between the two caged compounds with the use of blue light. Right, there is minimal functional cross-talk between the two caged compounds with the use of near-UV light. Recordings were made in the presence of TTX (1 μM), NMDA (DL-AP5, 100 μM) and GABA-B receptor (CGP-55845, 3 μM) and mGluR (JNJ-16259685, 1 μM; MPEP, 3 μM; LY341495, 30 nM) antagonists. For all cells, a single data point was collected at each flash duration and the data were averaged across cells.