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. 2017 Nov 8;12(11):e0187732. doi: 10.1371/journal.pone.0187732

Fig 6. Arbitrarily ordered two-color uncaging of glutamate and GABA on hippocampal CA1 neurons.

Fig 6

(a) In presence of dcPNPP-Glu and DEAC454-GABA, irradiation with 365 nm light (5 mW, 6 ms) evoked single action potentials. Preceding this stimulus with 470 nm light irradiation (10 mW, 1 ms) reversibly blocked the action potential. The 470 nm light stimulus alone did not evoke a detectable change in membrane potential (shunting inhibition). (b) 365 nm light irradiation (5 mW, 4 ms) at 1 Hz evoked multiple action potentials (left). Preceding the 2nd and 4th stimulus with 470 nm light irradiation (10 mW, 12 ms) reversibly blocked the action potentials selectively on these stimuli (middle). Removal of the blue flashes restored reliable action potential responses (right). DEAC454-GABA and dcPNPP-Glu were bath-applied at 27 μM and 310 μM, respectively. Recordings were made in the presence of NMDA (DL-AP5, 100 μM) and GABA-B receptor (CGP-55845, 3 μM) and mGluR (JNJ-16259685, 1 μM; MPEP, 3 μM; LY341495, 30 nM) antagonists.