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. 2017 Nov 8;12(11):e0187206. doi: 10.1371/journal.pone.0187206

Fig 1. Expression levels and localization of N-Myc protein in NB cell lines.

Fig 1

(A) Protein lysates from ACN, IMR-32, and LAN-1 cells were subjected to Western blot and probed with anti-N-Myc antibody. The blot was reprobed with anti-actin antibody as loading control. (B) ACN, IMR-32, and LAN-1 cells were cytospinned on Polysine™ slides and fixed. N-Myc protein nuclear localization was visualized by immunocytochemistry with anti-N-Myc antibody (60x magnification). Ctr = negative controls (20x magnification).