Table 2.
Primers used for amplification and sequencing.
| Locus | Primer | Orientation | Sequence (from 5′ to 3′) | References |
|---|---|---|---|---|
| benA | Bt2a | Forward | GGTAACCAAATCGGTGCTGCTTTC | Glass & Donaldson (1995) |
| T10 | Forward | ACGATAGGTTCACCTCCAGAC | O'Donnell & Cigelnik (1997) | |
| Bt2b | Reverse | ACCCTCAGTGTAGTGACCCTTGGC | Glass & Donaldson (1995) | |
| CaM | CF1L | Forward | GCCGACTCTTTGACYGARGAR | Peterson (2008) |
| CF1M | Forward | AGGCCGAYTCTYTGACYGA | Peterson (2008) | |
| cmd5 | Forward | CCGAGTACAAGGAGGCCTTC | Hong et al. (2006) | |
| CF4 | Reverse | TTTYTGCATCATRAGYTGGAC | Peterson (2008) | |
| cmd6 | Reverse | TTTYTGCATCATRAGYTGGAC | Hong et al. (2006) | |
| RPB2 | fRPB2-5F | Forward | GAYGAYMGWGATCAYTTYGG | Liu et al. (1999) |
| fRPB2ResF100 | Forward | TGAARTAYGCICTTGCYAC | Newly designed | |
| fRPB2-7CR | Reverse | CCCATRGCTTGYTTRCCCAT | Liu et al. (1999) | |
| fRPB2ResR950 | Reverse | CARTGYGTCCADGTRTGKGC | Newly designed | |
| ITS | ITS1 | Forward | TCCGTAGGTGAACCTGCGG | White et al. (1990) |
| NL4 | Reverse | GGTCCGTGTTTCAAGACGG | O'Donnell (1993) | |
| ITS4 | Reverse | TCCTCCGCTTATTGATATGC | White et al. (1990) |