Table 3. Analysis of the PPC Data for Full-Length and Truncated R67 DHFRs in 45 mM Na2HPO4, pH 8.0 Buffer with 0, 10, or 20% (w/w) Betaine.
| DHFR | [betaine] (%) | ΔV/V | αs10–40 (×10–4 K–1) | TM (°C) | αs10 (×10–4 K–1) | Δαs (×10–5 K–1) |
|---|---|---|---|---|---|---|
| full-length R67 DHFR | 0 | 0.0013 | 2.7 | 67.5 | 8.7 | 4.3 |
| 10 | 0.00077 | 3.1 | 70.0 | 9.7 | 4.8 | |
| 20 | 0.00048 | 3.2 | 72.4 | 8.9 | 3.3 | |
| truncated R67 DHFR | 0 | 0 | 9.7 | nda | 22.1 | nda |
| 10 | 0 | 8.2 | nda | 19.3 | nda | |
| 20 | 0 | 5.1 | nda | 14.5 | nda |
a
Not determined as there was no thermal denaturation transition in the PPC thermogram.