The Acyl-CoA levels and the mRNA expression of ACSL genes in prostate and hepatic cells. The prostate cells, PNT2 (normal prostate cells) and DU145 (prostate cancer cells), and hepatic cells, HepG2 (normal hepatic cells) and Hep3B (hepatic tumorigenic cells) were grown in the media recommended by ATCC. A) Cellular levels of acyl-CoAs measured by LC-MS/MS. “*” represents significant differences between nontumorigenic and tumorigenic cells (P < 0.05). B) Expression levels of ACSL1, 3, 4, 5 and 6 measured by RT-PCR. C) The spearman correlation between the level of individual acyl-CoA and the levels of each ACSL gene.