Skip to main content
. Author manuscript; available in PMC: 2017 Nov 9.
Published in final edited form as: J Med Chem. 2016 Mar 29;59(7):3112–3128. doi: 10.1021/acs.jmedchem.5b01894

Figure 2.

Figure 2

Crude RP-HPLC analytical chromatograms at 214 nm of 3 (mass of 961.6) in a gradient from 10% to 90% MeCN or MeOH in water containing 0.1 % trifluoroacetic acid at a flow rate of 1.5 mL/min over 35 minutes (5 to 20 minutes are shown) using an analytical Vydac C18 column (Vydac 218TP104). (A) Analytical HPLC trace in MeCN of crude peptide 3 after a three hour cleavage which shows only one major peak. A major impurity peak (mass of 685.4) is masked in this chromatogram. (B) Analytical HPLC trace in MeOH of crude peptide 3 after a three hour cleavage which identifies both the desired product and an impurity peak masked in MeCN chromatogram. (C) Co-injection of crude 3 from three hour cleavage with purified 1 (mass of 685.4) increases the intensity of the impurity peak demonstrating similar retention times. (D) A shorter cleavage time of 1.5 hours diminishes degradation product giving better crude peptide purity.