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. Author manuscript; available in PMC: 2018 Jul 26.
Published in final edited form as: J Am Chem Soc. 2017 Jul 17;139(29):9867–9875. doi: 10.1021/jacs.7b02958

Figure 3.

Figure 3

Efficient six-step protocol for functionalization of glass coverslips (a) and silicon-nitride tips (b) using hetero-bifunctional PEG. Tips and coverslips were activated using UV radiation and then silane-PEG functionalized in toluene at elevated temperature resulting in azide-derivatized coverslips and maleimide-derivatized AFM tips. After a series of solvent washes, the surfaces were dried. Biomolecules were covalently bound to coverslips using copper-free click chemistry, while thiol-modified streptavidin was covalently attached to maleimide-coated AFM tips. Finally, coverslips and tips were washed and stored in buffer at 4 °C until use.