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. Author manuscript; available in PMC: 2018 Jun 21.

Published in final edited form as: ACS Chem Neurosci. 2017 Feb 16;8(6):1262–1278. doi: 10.1021/acschemneuro.6b00399

A new cohort of male mice received a single treatment of saline vehicle control ( , n=10), 5 nmol CJL-1-14 ( , n=11), or 5 nmol CJL-1-87 ( , n=11) in the fasting paradigm used above (Figure 2B). CJL-1-87 treatment resulted in lowered refeeding food intake (A) corresponding to slower regain of body weight (B) measured manually. Measurements using an ECHOMRI-100H system showed no change in lean body mass percentage (C), but a significant decrease in body fat mass percentage was observed 6 h after treatment with CJL-1-87. (D). Mice were sacrificed 6 h post treatment and their trunk blood was analyzed using an Luminex Milliplex systems to examine insulin (E), C-peptide (F), leptin (G), GIP (H), ghrelin (I), IL-6 (J), and resistin (K). Hormone and cytokine levels are reported as pg per mL of plasma. Time 0 h was defined as the time of treatment. *p<0.05; **p<0.01; ***p<0.001 for CJL-1-87 compared to saline. +p<0.05; ++ p<0.01; +++p<0.001 for CJL-1-87 compared to CJL-1-14. % p<0.05; %% p<0.01; %%% p<0.001 for CJL-1-14 compared to saline.

Inline graphic — A new cohort of male mice received a single treatment of saline vehicle control ( , n=10), 5 nmol CJL-1-14 ( , n=11), or 5 nmol CJL-1-87 ( , n=11) in the fasting paradigm used above (Figure 2B). CJL-1-87 treatment resulted in lowered refeeding food intake (A) corresponding to slower regain of body weight (B) measured manually. Measurements using an ECHOMRI-100H system showed no change in lean body mass percentage (C), but a significant decrease in body fat mass percentage was observed 6 h after treatment with CJL-1-87. (D). Mice were sacrificed 6 h post treatment and their trunk blood was analyzed using an Luminex Milliplex systems to examine insulin (E), C-peptide (F), leptin (G), GIP (H), ghrelin (I), IL-6 (J), and resistin (K). Hormone and cytokine levels are reported as pg per mL of plasma. Time 0 h was defined as the time of treatment. *p<0.05; **p<0.01; ***p<0.001 for CJL-1-87 compared to saline. +p<0.05; ++ p<0.01; +++p<0.001 for CJL-1-87 compared to CJL-1-14. % p<0.05; %% p<0.01; %%% p<0.001 for CJL-1-14 compared to saline.