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. 2017 Nov 8;12(11):e0187831. doi: 10.1371/journal.pone.0187831

Fig 1. Effect of human (short) Link-N on human chondrocyte-like cells (CLCs).

Fig 1

GAG and DNA content (mean + SD) and histological evaluation of human CLC micro-aggregates treated with basal culture medium (control), supplemented with 10 ng/mL TGF-β1 (positive control), 1 μg/mL or 10 ng/mL human Link-N (LN), or 0.5 μg/mL or 5 ng /mL human short Link-N (sLN) for 28 days in normoxia (21% O2). (a) GAG content, (b) DNA content, (c) GAG content (incorporation in the micro-aggregate) corrected for DNA content, (d) total amount of GAGs released in the culture medium, (e) representative histological images of the Safranin O/Fast Green staining and collagen type I and II immunohistochemistry. *: p < 0.05; ● and ○: significantly different (p < 0.05) from all other conditions except for the bars with the same symbol; x: significantly different (p < 0.05) from all other conditions. n = 3 (in duplicates).