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. 2017 Nov 8;12(11):e0187831. doi: 10.1371/journal.pone.0187831

Fig 7. Effect of canine (short) Link-N on CD canine chondrocyte-like cells (CLCs).

Fig 7

GAG and DNA content (mean + SD) and histological evaluation of CD canine CLC micro-aggregates treated with basal culture medium (control), supplemented with 10 ng/mL TGF-β1, 1 μg/mL or 10 ng/mL canine Link-N (LN), or 0.5 μg/mL or 5 ng/mL canine short Link-N (sLN) for 28 days in hypoxia (5% O2). (a) GAG content, (b) DNA content, (c) GAG content (incorporation in the micro-aggregate) corrected for DNA content, (d) total amount of GAGs released in the culture medium, (e) representative histological images of the Safranin O/Fast Green staining and collagen type I and II immunohistochemistry. *: p < 0.05; x,xxx: significantly different (p < 0.05 and p < 0.001 respectively) from all other conditions. n = 6 (in duplicates).