Fig. 1. Characterization and fitness testing of synX strain.
(A) Design overview of synthetic chromosome X. (B) PCRTag analysis of synX (a pericentromeric region). Analysis of the complete set of PCRTags is shown in fig. S3. (C) Growth fitness of synX strain yYW0115 on various types of media. (D) Competitive growth assay of synX strain yYW0115 and native strain BY4741. Samples were analyzed by flow cytometry to quantify the ratio of RFP-positive to GFP-positive cells. Relative growth rate was calculated based on the ratio of synX to BY4741 at each time point.