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. Author manuscript; available in PMC: 2017 Nov 9.
Published in final edited form as: Science. 2017 Mar 10;355(6329):eaaf4706. doi: 10.1126/science.aaf4706

Fig. 3. PCRTag in FIP1 causes a growth defect.

Fig. 3

(A) The reverse (R) synthetic PCRTag recoded within YJR093C (FIP1) causes a growth defect. (B) FIP1 SYN PCRTag-R introduces a Rap1p recognition site. Percentages indicate similarity of FIP1 SYN PCRTag-R to known Rap1p binding sites. Red letters show differences between synthetic and native PCRTag-R sequences. (C) Growth assay and relative FIP1 RNA expression of codon-by-codon swap strains in FIP1 SYN PCRTag-R. Red letters represent SYN-specific bases; underlined letters represent restored WT codons. RNA level is quantified relative to WT FIP1.