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. Author manuscript; available in PMC: 2017 Nov 9.
Published in final edited form as: Neuron. 2016 Mar 3;89(6):1291–1304. doi: 10.1016/j.neuron.2016.01.041

Figure 6. Activation/Inhibition of ParvOT PVN Neurons Modulates Mechanical Threshold and Thermal Hot Latency in Animals Subjected to Complete Adjuvant Injection.

Figure 6

(A–A3) Scheme of the experimental procedure. The CAV2-Cre was injected in the SON and Cre-responding virus driving either (A2) ChR2 or (A3) hM4Di to achieve the expression of respective proteins in OT neurons of the PVN.

(B–B2) Mechanical thresholds and (B2) thermal hot latencies of naive animals before and after PVN-BL (ChR2, n = 6 and CNO, n = 10).

(C–C2) Mechanical thresholds and (C2) thermal hot latencies of the CFA-injected hindpaw (left graphs) and the contralateral hindpaw (right graphs). The effect of PVN-BL was assessed before, right after i.p. injection of OTR antagonist L-368,899 (1 mg/kg), and after its washout (n = 6). The effect of CNO (3 mg/kg) was measured 1 hr after i.p. injection and its 24 hr washout (n = 10). All results are expressed as average ± SEM. The statistical significance: * p < 0.05, ** p < 0.01, and one-way ANOVA followed by Tukey’s multiple comparison post hoc test.