Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 6;214(11):3347–3360. doi: 10.1084/jem.20170488

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Tian et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — Characterization of PBI-derived T cells. (A) Dot plot of FACS of lck:GFP⁺ cells from 16-dpf AGM-irradiated (left) and PBI-irradiated (middle) fish. GFP⁺ cells are gated from live cells, and autofluorescent cells are excluded by real-GFP⁺ gate. Three independent experiments (in each group, ∼20 fish are pooled together) are conducted. The cDNA are prepared from at least 250 lck:GFP⁺ cells isolated from 16-dpf AGM- and PBI-irradiated embryos and subjected to qPCR analysis. cDNA prepared from the whole 16-dpf embryos are used as a control. (B and C) Relative expression levels of tcrα and tcrδ in the AGM- and PBI-derived T cells. Three independent experiments are conducted. (D) Expression ratio of tcrδ to tcrα. The values are calculated on the basis of the expression level of tcrα and tcrδ relative to the internal control gene (elf1a). (E and F) Relative expression levels of cd4-1 and cd8α in the AGM- and PBI-derived T cells. Three independent experiments are conducted. Data are represented as mean ± SD. Unpaired, two-tailed t test was performed to determine significance. *, P < 0.05; **, P < 0.01; ***, P < 0.001. See also Fig. S4.