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. Author manuscript; available in PMC: 2018 Sep 11.
Published in final edited form as: Angew Chem Int Ed Engl. 2017 Aug 16;56(38):11404–11408. doi: 10.1002/anie.201704649

Figure 4.

Figure 4

Cytoplasmic delivery of membrane-impermeable phalloidin into cells following US-triggered rupture of nano-peptisomes. (a) Mean intracellular fluorescence (in relative fluorescence units; RFU) of A549 cells following delivery of labelled phalloidin from nano-peptisomes at varying US intensity and duty cycle (DC). (b) Live-cell image showing delivery of phalloidin (green) from nano-peptisomes, spatially resolved to a circular area of the A549 cell monolayer subjected to US. Cell nuclei are stained blue, scale bar = 200 µm. (c) Live A549 cells stained with the endosomal marker transferrin (red) following nano-peptisome mediated delivery of labelled phalloidin. Scale bar = 80 µm. (d) Average intracellular fluorescence of A549 cells upon delivery of labelled phalloidin from nano-peptisomes (NP) containing the RGD targeting motif, or the non-targeted control sequence RGE