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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Exp Eye Res. 2016 Nov 3;154:1–9. doi: 10.1016/j.exer.2016.11.003

Fig. 1.

Fig. 1

A: Fas knockdown 72 h after transfection with Fas siRNA. Lanes 1–3: control (arrow indicates Fas), lanes 4–6: Fas knockdown. The lanes show protein of cells from separate wells in the same experiment. This gel is representative of 12 knockdown experiments with similar results. B: Whole-cell K+ currents as a function of membrane potential. Currents in both control and Fas-knockdown cells were measured prior to and after 80 mJ/cm2 UVB exposure. UVB caused similar activation of K+ channels in both control and Fas knockdown cells. (Fas data are the mean ± SE of 12 cells. Control data are the mean ± SE of 11 cells). C: HCLE cells were exposed to 150 mJ/cm2 UVB. Cells were then incubated for 20 min after which intracellular K+ was measured by ion chromatography. Control cells lost 31% of intracellular K+ and cells treated with Fas siRNA lost 28%. Values marked * differ significantly from pre-UV conditions. (Mean ± SD, n = 12, ANOVA and SNK test, p < 0.05).