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. Author manuscript; available in PMC: 2017 Nov 9.
Published in final edited form as: J Am Chem Soc. 2017 Jul 24;139(30):10196–10199. doi: 10.1021/jacs.7b05196

Table 2.

Screening of focused library for allylic oxidation

Entry Catalysta Active-Site Ala Substitutions Desired/Undesiredb
1 P450BM3 – 0.86
2 2A1 L75A, L181A, L437A 0.37
3 4H5 L75A, L177A, L181A 2.1
4 8C7 L75A, L181A 2.8
a

See supporting information for full list of mutations.

b

Approximated by separating the products on UHPLC-MS and comparing ion count of C-7 oxidation product to all other mono-oxygenation products (m/z = 286). See supporting information for more details.