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. Author manuscript; available in PMC: 2018 Oct 15.
Published in final edited form as: J Immunol. 2017 Sep 20;199(8):2823–2833. doi: 10.4049/jimmunol.1500832

Figure 6. NLRP10−/− and WT myeloid cells have similar migration toward chemoattractants and leishmanicidal activity.

Figure 6

(A-C) Transwell migration assays were performed with (A) PMNs, (B) monocytes, and (C) bone marrow-derived DCs. The WT and NLRP10−/− monocytes were isolated from bone marrow and analyzed in a transwell migration assay for their migration toward zymosan activated serum or MCP-1. (B) WT and NLRP10−/− PMNs were isolated from bone marrow and tested in a transwell assay for migration toward zymosan activated serum or heat-killed S. aureus. (C) WT or NLRP10−/− bone marrow-derived DCs were assayed in a transwell assay for their migration toward CCL12, CCL19, or CCL21. D-E) To test their intracellular survival, serum opsonized, metacyclic L. major WT Friedlin strain promastigotes were added to WT or NLRP10−/− BMDMs at a parasite to macrophage ratio of 5:1 . (D) The percent infection and (E) parasites/100 macrophages were quantified microscopically at the indicated times post infection. Results were compiled from 3 independent experiments, each with triplicate slides, and expressed as the mean ± SEM.