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. Author manuscript; available in PMC: 2017 Nov 9.
Published in final edited form as: Biochemistry. 2017 Jan 18;56(4):647–656. doi: 10.1021/acs.biochem.6b00569

Fig. 3. Acetylation does not enhance activity of DNA Polymerase β on nucleosome substrates.

Fig. 3

The ability of Pol β or acetylated Pol β to extend DNA templates assembled into nucleosomes was assessed. Increasing concentrations of Pol β or acPol β polymerase (0, 25, 50, 100, and 200 fmol) were incubated with nucleosomes (1 fmol) containing the −5/−6, −11/−12, +49/+50 or +54/+55 templates (A–D, respectively) and products analyzed as in Fig. 2. Products of Pol β extension (25 fmol) on naked templates is shown in lanes marked “DNA + Pol β.”