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. Author manuscript; available in PMC: 2017 Nov 9.
Published in final edited form as: Biochemistry. 2017 Jan 18;56(4):647–656. doi: 10.1021/acs.biochem.6b00569

Fig. 4. HMGB1 modestly stimulates Pol β DNA activity only on the −5/−6 gapped DNA template. A.

Fig. 4

A–D. The −5/−6, −11/−12, +49/+50 or +54/+55 DNA templates were incubated without (Lanes 1) or with 25 fmol of Pol β in the absence (lanes 2) or presence of increasing amounts of HMGB1 (Lanes 3–6). (Note that in D, the 5 fmol HMGB1 lane was omitted). Products were analyzed as in Fig. 1. E. The fraction of templates extended by Pol β was determined by quantification of total extension products/total lane density, normalized to that found in the absence of HMGB1 for each experiment and the results averaged and plotted for 1 and 10 fmol HMGB1. Bars indicate +/− standard error, N= 2–4. F. Scans of the 0 and 0.5 fmol HMGB1 lanes in the gels shown in A–D (black and red traces, respectively), showing the increase in extension products observed only for the −5/−6 template.