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. Author manuscript; available in PMC: 2018 Oct 11.
Published in final edited form as: Cell Host Microbe. 2017 Sep 21;22(4):543–551.e4. doi: 10.1016/j.chom.2017.08.008

Figure 3. O-acetylation of S. aureus PGN limits the induction of pro-inflammatory cytokines critical for protective memory.

Figure 3

(A) BMDC were stimulated with WT (S. aureus SA113), ΔoatA (isogenic SA113 mutant) or 1:1 mixture of WT and ΔoatA at MOI of 10. Supernatants were harvested at 24h for cytokine determination. Data are representative of 3 experiments. See also Figure S3.

(B)–(C) Mice (n=5) were infected with WT S. aureus (2×107) or WT + ΔoatA (107 CFU of each) at d0, 7 and 14. (B) Two days after the last infection, splenocytes were harvested from the mice for determination of cytokine levels. (C) Seven days after the last infection, splenocytes were harvested and stimulated with heat-killed WT S. aureus and cytokines were measured after 3d.

For (B) and (C), each data point represents an individual mouse, and mean ± SEM are shown for all data. Data analysis was performed using Student’s t-test (A) and using Mann-Whitney U test for (B) and (C). *p<0.05, ***p<0.001.