Figure 1. Interspecies Rat-Mouse Chimeras Derived from Rat PSCs.
(A) Rat-mouse chimeras generated by rat ESCs (DAC2). Left, an E18.5 rat-mouse chimeric fetus. Red, hKO-labeled rat cells. Right, a 12-month-old (top) and 24-month-old (bottom) rat-mouse chimera.
(B) Chimera forming efficiencies with rat ESC lines (DAC2 and DAC8) and rat iPSC lines (SDFE and SDFF). n, number of embryo transfers.
(C) Representative fluorescence images showing hKO-labeled rat ESCs (DAC2) contributed to different tissues in the 24-month-old rat-mouse chimera. Red, hKO-labeled rat cells. Blue, DAPI. Scale bar, 100 μm.
(D) Representative immunofluorescence images showing the expression of aging-related histone marks, including H3K9me3 and H4K20me3, in the kidney tissue of neonatal and 24-month-old chimeras. Scale bar, 10 μm.
(E) Levels of chimerism of rat ESCs (DAC2) in different tissues of the 24-month-old rat-mouse chimera. Error bars indicate SD.
(F) Rat iPSCs (SDFE) contributed to the neonatal mouse gall bladder. Left, bright-field (top) and fluorescence (bottom) images showing a neonatal mouse gallbladder contained cells derived from rat iPSCs. White arrowheads indicate the gallbladder. Right, representative immunofluorescence images showing the expression of a gallbladder epithelium marker (EpCAM) by rat cells. Red, hKO-labeled rat cells; blue, DAPI. Scale bar, 50 μm.