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. Author manuscript; available in PMC: 2018 Jan 26.
Published in final edited form as: Cell. 2017 Jan 26;168(3):473–486.e15. doi: 10.1016/j.cell.2016.12.036

Figure 6. Chimeric Contribution of hiPSCs to Post-implantation Pig Embryos.

Figure 6

(A) Representative bright field (left top) fluorescence (left bottom and middle) and immunofluorescence (right) images of GFP-labeled FAC-hiPSCs derivatives in a normal size day 28 pig embryo (FAC #1). Scale bar, 100 μm.

(B) Representative immunofluorescence images showing chimeric contribution and differentiation of FAC-hiPSCs in a normal size, day 28 pig embryo (FAC #1). FAC-hiPSC derivatives are visualized by antibodies against GFP (top), TUJ1, SMA, CK8 and HuNu (middle). (Bottom) Merged images with DAPI. Insets are higher magnification images of boxed regions. Scale bar, 100 μm.

(C) Representative gel images showing genomic PCR analyses of pig embryos derived from blastocyst injection of 2iLD-iPSCs (surrogates #8164 and #20749) and FAC-hiPSCs (surrogates #9159 and #18771) using a human specific Alu primer. A pig specific primer Cyt b was used for loading control. nc, negative control with no genomic DNA loaded. pc, positive controls with human cells. Pig 1D, 1G, and 1I, pig controls. ID, surrogate and pig embryos.

See also Figure S5 and Table S2.