Skip to main content
. Author manuscript; available in PMC: 2018 Sep 19.
Published in final edited form as: Immunity. 2017 Sep 12;47(3):498–509.e6. doi: 10.1016/j.immuni.2017.08.007

Figure 5. Overexpression of SIDT2 enhances endosomal escape of poly(I:C).

Figure 5

(A) Doxycycline-inducible SIDT2-mCherry DC2.4 cells were treated with poly(I:C)-fluorescein for 1 h in the presence or absence of doxycycline and imaged by confocal microscopy. Scale bar = 10 μm (B) The proportion of each cell occupied by punctate poly(I:C)-fluorescein staining was individually quantified for >60 cells across multiple fields of view, and plotted as mean ± SEM. (C) Internalisation of poly(I:C)-fluorescein by SIDT2-mCherry+ and SIDT2-mCherry− cells after 1 h at either 4°C or 37°C was assessed via flow cytometry. Data are representative of 3 independent experiments. ****P < 0.0001. See also Figure S5.