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. Author manuscript; available in PMC: 2018 Sep 19.
Published in final edited form as: Immunity. 2017 Sep 12;47(3):498–509.e6. doi: 10.1016/j.immuni.2017.08.007

Figure 6. SIDT2 is important for RLR- but not TLR-mediated IFN production in response to extracellular dsRNA.

Figure 6

(A) Sidt2+/+ and Sidt2−/− mice were injected i.p with 50 μg poly(I:C) per 25 g body weight (n=7), and serum IFNβ measured at 3 h via ELISA. (B) Sidt2+/+ and Sidt2−/− mice (n=8) were injected i.p. with 300 μg poly(A:U) per 25 g body weight and serum IFNβ measured at 3 h via ELISA. (C) Sidt2+/+ and Sidt2−/− mice (n=8) were injected i.p. with 50 μg poly(I:C) per 25 g body weight and serum IFNλ measured at 3 h via ELISA (n=8). Data are plotted as mean ± SEM. (D) BMDMs from Sidt2+/+ and Sidt2−/− were stimulated with 10 μg/ml poly(I:C) for the indicated times and pIRF3Ser386 and total IRF3 was assessed via immunoblotting. (E) Sidt2+/+ and Sidt2−/− MEFs were transfected with MAVS-YFP, treated with poly(I:C) for 1 h, and MAVS aggregation assessed via confocal microscopy. Scale bar = 40 μm. (F) Individual cells (>150 per condition) were scored for the appearance of MAVS aggregates. Data is representative of 3 independent experiments and error bars are plotted as mean ± SEM. * P < 0.05, n.s. = not significant, n.d = not detected.