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. Author manuscript; available in PMC: 2018 Oct 5.
Published in final edited form as: Mol Cell. 2017 Sep 14;68(1):118–129.e5. doi: 10.1016/j.molcel.2017.08.014

Figure 1.

Figure 1

Auxin-dependent depletion of yeast TFIID subunits. (A) Listed at left are yeast TFIID subunits in lobe C, the shared or similar TFIID/SAGA core subunits and other auxiliary subunits. * indicates subunits tagged with the 3xV5-IAA7 degron tag and # indicates subunits tagged with FRB for anchor-away. Blue indicates subunits shared with SAGA and Orange indicates that SAGA contains a related but SAGA-specific subunit. Shown at right is Western analysis of cells treated with 3-IAA for the indicated time. Western blot was probed with anti V5 antibody. (B) Analysis of TFIID subunits after Taf depletion. After 30 min treatment with 3-IAA, whole cell extracts were made and immune precipitated with anti Taf4 antibody. Shown is quantitative Western analysis of the IP, probing for Tafs 1, 3, 4, 12 and TBP. * indicates a non-specific signal observed with the anti Taf1 antibody.