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. Author manuscript; available in PMC: 2017 Dec 15.
Published in final edited form as: Cell. 2016 Dec 15;167(7):1719–1733.e12. doi: 10.1016/j.cell.2016.11.052

Figure 3. Establishment of In Vivo Induction Protocol in 4F Mice.

Figure 3

(A) Body weight of 4F mice upon continuous administration of doxycycline (−Dox n = 11; +Dox n = 26).

(B) Survival of 4F mice upon continuous administration of doxycycline (−Dox n = 11; +Dox n = 26). ***p < 0.0005 according to log-rank (Mantel-Cox) test.

(C) Schematic representation of cyclic doxycy-cline administration protocol.

(D) qPCR analysis of Oct4, Sox2, Klf4, and c-Myc in blood samples of 4F mice after 2 days of doxycycline administration. **p < 0.005, ***p < 0.0005, and ****p < 0.0001 according to one-way ANOVA with Bonferroni correction.

(E) Teratomas (arrows) in 4F mice carrying two copies of OSKM and rtTA cassette after 8 weeks of cyclic doxycycline administration.

(F) Histological analysis of teratoma with ectoderm, mesoderm, and endoderm. Scale bar, 50 μm.

Data are presented as mean ± SEM. See also Figure S3.